Capillary electrophoresis
Capillary electrophoresis (CE) is a separation and analytical method of which the differential migration rates of sample components is caused by an applied electrical field within a capillary, small-diameter polyimide coated fused silica capillary tube usually. “On-column” UV spectrometric or fluorescence analysis is usually used for detection of sample components through a “window” in the capillary. CE is a powerful technique having a wide range of applications including; analysis of proteins, peptides, chiral compounds, pharmaceuticals, inorganic ions, and specially sizing and characterization of nanomaterials.
Fnm's Capillary electrophoresis (CE) is designed based on its minimal sample and solvent requirements, rapid analysis time and high efficiency and resolution useful in many laboratories. It covers a broad range of applications in a wide variety of industries. Some of its main application fields include: i) food analysis, ii) pharmaceutical analysis, iii) bioanalysis, iv) environmental pollutants analysis, and v) nanomaterial analysis.
Principle of operation
Capillary electrophoresis (CE) is a family of related separation techniques that use narrow-bore fused silica capillaries to separate a complex array of large and small molecules. High voltages are used to separate molecules based on differences in charge, size and hydrophobicity. Injection into the capillary is accomplished by immersing the end of the capillary into a sample vial and applying pressure, vacuum or voltage. Separated solutes are quantitatively detected at the capillary outlet by high sensitive optical system based on direct or indirect UV absorbance.
Specification
FEATURES AND BENEFITS
• High separation efficiency
• Short analysis time
• Low sample and electrolyte consumption
• Low waste generation
• User friendliness: Complete control of the instrument from a PC
Powerful software package
• Increased flexibility in performing analyses of various complexity
• Any kind of complex runs are possible including those with pre-programming of changes in analysis conditions
• Customized report, data export to other programs
Extended instrumental options
• Spectra scanning facilitates peak identification
• Broad range of controlled pressure injection permits analysis of viscous samples
Analytical characteristics
Power voltage range
• Adjustable 1 to 20 kV high voltage power supply
• Operation under constant voltage
• Show current (µA)
Autosampler
• A 16-position carousel.
• All vials are randomly accessible from electrodes end of capillary.
Detectors
• CEP 1000 is equipped with variable wavelength UV-detector
• Wavelength range 200 - 1100 nm.
Light source
• Halogen lamp (Visible and near IR)
• Deuterium lamp (UV light)
Vials
• Standard 1.5 ml
• Minimum sample volume 500 µl.
Injection modes
• Controlled pressure profile injection with variable peak pressure, programmable peak pressure range being 20 - 100 mbar
• Electrokinetic (1-5kV)
• Programmable injection time
Pressure system
• Programmable with 20-100 mbar for injection, washing and flushing with maximum 1 bar.
Analysis
• Voltage range settable from 1 to 20 kV
• Current from 0 to 500 mA
Software features
• Real time electropherogram visualization
• electropherogram data processing
• Computation of electrophoresis system parameters
• Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors
• Wave scan
Safety features
Disabling high voltage function:
• Over current limit
• Earth detection system
• Arc detection system
• Safety sensors at doors and cover
Areas of application
• Food analysis
• Environmental pollutants analysis
• Chemical industry
• Pharmaceutical analysis
• Bioanalysis
Warranty
• All of the CEP 1000 equipment is covered by a 12-months warranty.
Services
Upon request installation and commissioning of CEP 1000 systems can be carried out on Customer's site by our service engineers. Consultations and initial training of attendance personnel with due account of Customer's specific needs are performed. Spare parts are delivered and repair is made upon Customer's request (free within the warranty period).