ISI Papers With Our Products
Title: Fabrication and Characterization of Nanofibrous Poly (L-Lactic Acid)/Chitosan-Based Scaffold by Liquid–Liquid Phase Separation Technique for Nerve Tissue Engineering
Journal: Molecular Biotechnology
Author: 1. Arian Ehterami, 2. Masoomeh Masoomikarimi, 3,4. Farshid Bastami, 5. Moslem Jafarisani, 6. Morteza Alizadeh, 6,8,9. Majid Salehi, 7. Mohsen Mehrabi
Year: 2022
Address: 1. Institute for Regenerative Medicine, University of Zurich, Zurich, Switzerland
2. Depertment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Oral and Maxillofacial Surgery, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University
of Medical Sciences, Tehran, Iran
5. Department of Clinical Biochemistry, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
6. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
7. Department of Medical Nanotechnology, School of Medicine, Shahroud University of Medical Sciences,
Shahroud, Iran
8. Sexual Health and Fertility Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
9. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
Abstract: Fabrication method is one of the essential factors which directly affect on the properties of scaffold. Several techniques have been well established to fabricate nanofibrous scaffolds such as electrospinning. However, preparing a three-dimensional (3-D) interconnected macro-pore scaffold essential for transporting the cell metabolites and nutrients is difficult using the
electrospinning method. The main aim of this study was developing a highly porous scaffold by poly (L-lactic acid) (PLLA)/
chitosan blend using liquid–liquid phase separation (LLPS) technique, a fast and cost–benefit method, in order to use in
nerve tissue engineering. In addition, the effect of different polymeric concentrations on morphology, mechanical properties, hydrophilicity, in vitro degradation rate and pH alteration of the scaffolds were evaluated. Moreover, cell attachment, cell viability and cell proliferation of scaffolds as candidates for nerve tissue engineering was investigated. PLLA/chitosan blend not only had desirable structural properties, porosity, hydrophilicity, mechanical properties, degradation rate and pH alteration but also provided a favorable environment for attachment, viability, and proliferation of human neuroblastoma cells, exhibiting significant potential for nerve tissue engineering applications. However, the polymeric concentration in blend fabrication had influence on both characteristics and cell responses. It concluded that PLLA/chitosan nanofibrous 3-D scaffold fabricated by LLPS method as a suitable candidate for nerve tissue engineering.
Keywords: Thermally induced phase separation (TIPS), Poly (l-lactic acid), Chitosan, Peripheral nerve regeneration,
Nerve tissue engineering
Application: Tissue Engineering
Product Model 1: Syringe Pump
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URL: #https://link.springer.com/article/10.1007/s12033-021-00346-3#