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Title: Fabrication of 3D hybrid scaffold by combination technique of electrospinning-like and freeze-drying to create mechanotransduction signals and mimic extracellular matrix function of skin
Journal: Materials Science and Engineering: C
Author: 1. Azadeh Izadyari Aghmiuni, Azim Akbarzadeh Khiyavi, 2,3. Saeed Heidari Keshel, 4,5. Farshid Sefat
Year: 2021
Address: 1. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran. 2. Medical Nanotechnology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4. Department of Biomedical and Electronics Engineering, School of Engineering, University of Bradford, Bradford, UK. 5. Interdisciplinary Research Centre in Polymer Science & Technology (IRC Polymer), University of Bradford, Bradford, UK.
Abstract: Fabrication of extracellular matrix (ECM)-like scaffolds (in terms of structural-functional) is the main challenge in skin tissue engineering. Herein, inspired by macromolecular components of ECM, a novel hybrid scaffold suggested which includes silk/hyaluronan (SF/HA) bio-complex modified by PCP: [polyethylene glycol/chitosan/poly(ɛ-caprolactone)] copolymer containing collagen to differentiate human-adipose-derived stem cells into keratinocytes. In followed by, different weight ratios (wt%) of SF/HA (S1:100/0, S2:80/20, S3:50/50) were applied to study the role of SF/HA in the improvement of physicochemical and biological functions of scaffolds. Notably, the combination of electrospinning-like and freeze-drying methods was also utilized as a new method to create a coherent 3D-network. The results indicated this novel technique was led to ~8% improvement of the scaffold’s ductility and ~17% decrease in mean pore diameter, compared to the freeze-drying method. Moreover, the increase of HA (>20wt%) increased porosity to 99%, however, higher tensile strength, modulus, and water absorption% were related to S2 (38.1, 0.32 MPa, 75.3%). More expression of keratinocytes along with growth pattern similar to skin was also observed on S2. This study showed control of HA content creates a microporous-environment with proper modulus and swelling%, although, the role of collagen/PCP as base biocomposite and fabrication technique was undeniable on the inductive signaling of cells. Such a scaffold can mimic skin properties and act as the growth factor through inducing keratinocytes differentiation.
Keywords: ECM components, Skin tissue engineering, Composite scaffolds, Fabrication techniques, Keratinocytes.
Application: Tissue Engineering, Scaffold
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URL: #https://www.sciencedirect.com/science/article/pii/S0928493120336717#