ISI Papers With Our Products

Title: In vitro and in vivo evaluation of silk fibroin-hardystonite-gentamicin nanofibrous scaffold for tissue engineering applications
Journal: Polymer Testing
Author: 1,2. Zhina Hadisi, Tavia Walsh, Erik Pagan, Mohsen Akbari, 3. Hamid Reza Bakhsheshi-Rad, 4. Saeed Farzad Mohajeri, Hossein Gholami, 4,5. Mohammad Mehdi Dehghan, 6. Anahita Diyanoush,
Year: 2020
Address: 1. Laboratory for Innovations in Micro Engineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, Canada 2. Center for Advanced Materials and Related Technologies (CAMTEC), University of Victoria, Victoria, Canada 3. Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran 4. Institute of Biomedical Research, University of Tehran, Tehran, Iran 5. Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 6. Department of polymer Engineering, Amirkabir University of Technology, Tehran, Iran
Abstract: Designing advanced biomaterials with regenerative and drug delivering functionalities remains a challenge in the field of bone and tissue engineering. In this paper we present the design, development, and a use case of an electrospun nano-biocomposite scaffold composed of silk fibroin (SF), hardystonite (HT), and gentamicin (GEN). The fabricated SF nanofiber scaffolds provide mechanical support while HT acts as a bioactive and drug carrier, on which GEN is loaded as an antibacterial agent. Antibacterial zone of inhibition (ZOI) results indicate that the inclusion of 3-6 wt% GEN significantly improves the antibacterial performance of the scaffolds against Gram-negative Escherichia coli (E. coli) and Grampositive Staphylococcus aureus (S. aureus) bacteria, with an initial burst release of 10-20% and 72-85% total release over 7 days. The release rate of stimulatory silicon ions from SF-HT scaffolds reached 94.53±5ppm after 7 days. Cell studies using osteoblasts show that the addition of HT significantly improved the cytocompatibility of the scaffolds. Angiogenesis, in vivo biocompatibility, tissue vascularization, and translatability of the scaffolds were studied via subcutaneous implantation in a rodent model over 4-weeks. When implanted subcutaneously, the GEN-loaded scaffold promoted angiogenesis and collagen formation, which suggests that the scaffold may be highly beneficial for further bone tissue engineering applications.
Keywords: Drug delivery, in vivo, subcutaneous implant, nanofiber, electrospinning, silk fibroin
Application: Tissue Engineering, Scaffold, Drug Delivery, Antibacterial Properties
Product Model 1: Electroris
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/abs/pii/S0142941820300623#