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Title: Herbal extract incorporated chitosan based nanofibers as a new strategy for smart anticancer drug delivery system: an in vitro model
Journal: World Cancer Research Journal
Author: 1. A. JAFARI, 2. S.J. SEYYED TABAEI, 3,4. M. RAHIMI, 5. S. TARANEJOO, 6,7. M. GHANIMATDAN
Year: 2020
Address: 1. Student Research Committee, Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3. Department of Parasitology and Mycology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran 4. Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran 5. Wellman Center for Photomedicine, Massacheussetts General Hospital Bartlett Hall 708, Harvard-MIT Division of Health Sciences and Technology (HST), Harvard, MA, USA 6. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran 7. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract: Objective: Despite the anticancer effect of Berberine (BBR), low aqueous solubility and poor gastrointestinal absorption can make its therapeutic usage diffcult. However, chitosan/ polyethylene oxide (CH/PEO) nanofbers scaffold eliminate this problem. This study has been conducted to recognize CH/PEO/BBR nanofbers effect on cancer cell lines. Materials and Methods: CH/PEO solution was prepared at different ratios for achieving optimal nanofbers. CH/PEO/BBR nanofbers were provided via electrospinning. Internal structure and 3-D morphology of fbers were studied using TEM and AFM, respectively. Functional groups were analyzed by a Fourier Transform Infrared (FTIR) spectroscopic device. Characterization of electrospun nanofbers was done by SEM. BBR released from nanoscaffolds was detected within 2 weeks by a UV-Visible device. The growth and proliferation of human breast cancer cell lines (MDA-MB-468, BT474 and MCF7), human HeLa cervical cancer cells and fbroblast cells in cultured medium were investigated by an inverted microscope. The cytotoxic effect of CH/PEO/BBR nanofbers against mentioned cell lines was characterized by MTT assay. Statistical analysis was done by SPSS-18 software. p<0.05 was considered as signifcant. Results: Nanoscaffolds containing 0.5-20 wt.% BBR concentrations inhibited cell growth compared to the control group in HeLa, BT474, MCF7 and MDA-MB-468 cell lines. The cell viability of cancer cell lines was signifcantly decreased after exposure with CH/PEO/BBR in a time dependent manner (HeLa, BT474, MCF7 (p=0.000) and MDA-MB-468 (p=0.001)). Conclusions: Our results suggested that CH/PEO/BBR nanofber has the potential to be developed as co-chemotherapeutic agent for human breast and cervical cancer therapy. However, its molecular mechanisms need to be further explored.
Keywords: Chitosan/Polyethylene oxide, Cancer, Drug delivery system, Natural compounds, Therapeutic agent
Application: Drug Delivery
Product Model 1: Electroris
Product Model 2:
URL: #https://www.wcrj.net/wp-content/uploads/sites/5/2020/01/e1462-Herbal-extract-incorporated-chitosan-based-nanofibers-as-a-new-strategy-for-smart-anticancer-drug-delivery-system-an-in-vitro-model.pdf#