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Title: Cell attachment effects of collagen nanoparticles on crosslinked electrospun nanofibers
Journal: The International Journal of Artificial Organs
Author: 1. Fereshteh Ziaei Amiri, Zaiddodine Pashandi, Mohammad Javad Mirzaie Parsa, Hossein Ghanbari, Reza Faridi-Majidi, 2. Nasrin Lotfibakhshaiesh
Year: 2020
Address: 1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. Department of Tissue Engineering and Applied Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract: Since collagen is naturally a main extracellular matrix protein, it has been applied widely in skin’s tissue engineering scaffolds to mimics the characteristics of extracellular matrix for proper transplantation of living cells. However, there are challenges that come with application of this natural polymer such as high solubility in aqueous environments which requires further consideration such as chemically cross-linking in order to stabilization. But these treatments also affect its functionality and finally cellular behaviors on scaffold. In this research we evaluated the suitability of collagen nanofibers versus collagen nanoparticles for cell adhesion and viability on glutaraldehyde cross-linked scaffolds. Applying a dual-pump electrospinning machine a blend PCL-Gelatin from one side and collagen nanofibers or collagen nanoparticles from the other side were collected on the collector. The fabricated scaffolds were characterized by scanning electron microscopy, contact angle, and mechanical analysis. The cell viability, adhesion and morphology were studied respectively using MTT assay, hoechst staining and scanning electron microscopy. The results indicated significantly improvement of cell viability, adhesion and better spreading on scaffolds with collagen nanoparticles than collagen nanofibers. It seems changes in surface morphology, viscoelastic moduli and swelling ability following cross-linking with glutaraldehyde in scaffold with collagen nanoparticles are still favorable for cellular proliferation. Based on these results, in the case of glutaraldehyde cross-linking, application of collagen nanoparticles rather than collagen nanofibers in tissue regeneration scaffolds will better mimic the extracellular matrix characteristics; and preserve the viability and adhesion of seeded cells.
Keywords: Collagen nanoparticle, glutaraldehyde, nanofiber surface, electrospun scaffold, tissue regeneration
Application: Tissue Engineering, Scaffold
Product Model 1: Dual Pump Electroris
Product Model 2:
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