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Title: Tailored PCL scaffolds as skin substitutes using sacrificial PVP fibers and collagen/chitosan blends
Journal: International Journal of Molecular Sciences
Author: 1. Samira Nokhasteh, 1,2. Ali Reza Sadeghi-avalshahr, 1,3. Amir Mahdi Molavi, 4. Najmeh Mohammad-pour, 5. Mohammad Sadeghi
Year: 2020
Address: 1. Department of Materials Research, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad 91775-1376, Iran 2. Department of Biomaterials, College of Biomedical Engineering, Amirkabir University of Technology, Tehran 159163-4311, Iran 3. Materials Engineering Department, Tarbiat Modares University, Tehran 14115-111, Iran 4. Department of Stem Cell and Regenerative Medicine Research, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad 91775-1376, Iran 5. Department of Mechanical Engineering, Tarbiat Modares University, Tehran 14115-111, Iran
Abstract: Electrospinning is a versatile technique for fabrication of made-on-purpose biomimetic scaffolds. In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh. After aminolysis, a blend of collagen/chitosan was grafted on the surface. Physicochemical characterizations as well as in vitro evaluations were conducted using different methods. Successful cell infiltration into samples was observed. It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 µm before immersion in water to 33.55 µm after immersion in water for 24 h). Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT). Viability evaluation through MTT assay for HDF cells did not reveal any cytotoxic effects. Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend. The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
Keywords: collagen, chitosan, aminolysis, electrospinning, grafting, wound healing
Application: Wound Dressing
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