ISI Papers With Our Products

Title: Polyurethane/Gelatin nanofiber neural guidance conduit in combination with resveratrol and schwann cells for sciatic nerve regeneration in the rat model
Journal: Fibers and Polymers
Author: 1,2. Majid Salehi, 3. Arian Ehtrami, 4. Sepanta Hosseinpour, 4,5. Farshid Bastami, 6. Ahmad Vaez, Arash Goodarzi, Saeed Farzamfar, 7. Hamid Vahedi, 8. Mostafa Rahvar
Year: 2019
Address: 1. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud 3614773955, Iran 2. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud 3614773955, Iran 3. Department of Mechanical and Aerospace Engineering, Science and Research Branch, Islamic Azad University, Tehran 1477893855, Iran 4. Dental Research Center, Research Institute of Dental Science, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran 1983963113, Iran 5. Oral and Maxillofacial Surgery Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran 1983963113, Iran 6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran 7. Clinical Research Development Unit, Imam Hossein Hospital, Shahroud University of Medical Sciences, Shahroud 3614773955, Iran 8. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran
Abstract: Peripheral nerve injury is a serious challenge which influences 2.8 percent of trauma patients. Tissue engineering of peripheral nerves mainly focuses on axonal regeneration via various nerve guides. The aim of this study is to evaluate a novel polyurethane (PU)/gelatin nanofibers (GNFs) conduit’s potential combination with resveratrol (RVT) for sciatic nerve regeneration in the rat. Platelet-rich plasma (PRP) was used as a carrier for RVT. Different tests like contact angle, tensile strength etc. was used to evaluate properties of PU/GNFs conduits. In addition, the electron microscopy, MTT assay, and DAPI staining revealed its compatibility with Schwann cells. 24 male Wistar rats were allocated into four groups (n=6) (1) PU/GNF/PRP/Schwann cell, 2) PU/GNF/Schwann cell/PRP/RVT, 3) Positive control, and 4) Negative control in order to assess sciatic functional index (SFI), hot plate latency, electromyographical (EMG), the percentage of wet weight-loss of gastrocnemius muscle and histopathological studies using hematoxylin-eosin staining. The results represented sciatic functional index (SFI), hot plate latency, electromyographical improved significantly in group 1 and 2 compared to the negative control group. Histopathological findings showed remarkable improvements in myelin sheath regeneration and fibers condition in group 1 and 2 compared to the negative control group. Group 2 showed more resemblance to the normal sciatic nerve, with well-arranged fibers and an intact myelin sheath. This study successfully applied PU/GNFs/PRP/RVT conduits as a potential biocompatible nerve guide with proper mechanical properties, biocompatibility, and biodegradability that enhanced injured sciatic nerve’s recovery rate.
Keywords: Polyurethane, Gelatin nanofibers, Resveratrol, Neural guidance conduit, Schwann cells
Application: Tissue Engineering
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URL: #https://link.springer.com/article/10.1007/s12221-019-8939-3#