ISI Papers With Our Products
Title: Electrospun Plla Nanofiber Scaffolds for Bladder Smooth Muscle Reconstruction
Journal: International urology and nephrology
Author: 1. Mohammad Ali Derakhshan, Hossein Ghanbari, Reza Faridi‑Majidi, 1,2. Gholamreza Pourmand, 3. Rassoul Dinarvand, Mohammad Naji, 5. Jafar Ai
Year: 2016
Address: 1. Department of Medical Nanotechnology, School
of AdvancedTechnologies in Medicine, Tehran University
of MedicalSciences, 1417755469 Tehran, Iran
2. Urology Research Center, Tehran University of Medical
Sciences, Tehran, Iran
3. Department of Pharmaceutics, Faculty of Pharmacy, Tehran
University of Medical Sciences, Tehran, Iran
4. Urology and Nephrology Research Center (UNRC), Shahid
Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Tissue Engineering, School of Advanced
Technologies in Medicine, Tehran University of Medical
Sciences, Tehran, Iran
Abstract: Purpose Urinary bladder may encounter several pathologic
conditions that could lead to loss of its function.
Tissue engineering using electrospun PLLA scaffolds is
a promising approach to reconstructing or replacing the
problematic bladder.
Methods PLLA nanofibrous scaffolds were prepared utilizing
single-nozzle electrospinning. The morphology and
distribution of fiber diameters were investigated by scanning
electron microscopy (SEM). Human bladder smooth
muscle cells (hBSMCs) were isolated from biopsies and
characterized by immunocytochemistry (ICC). Then, the
cells were seeded on the PLLA nanofibers and Alamar Blue
assay proved the biocompatibility of prepared scaffolds.
Cell attachment on the nanofibers and also cell morphology
over fibrous scaffolds were observed by SEM.
Results The results indicated that electrospun PLLA scaffold
provides proper conditions for hBSMCs to interact and
attach efficiently to the fibers. Alamar Blue assay showed the compatibility of the obtained electrospun scaffolds
with hBSMCs. Also, it was observed that the cells could
achieve highly elongated morphology and their native
aligned direction besides each other on the random electrospun
scaffolds and in the absence of supporting aligned
nanofibers.
Conclusion Electrospun PLLA scaffold efficiently supports
the hBSMCs growth and alignment and also has
proper cell compatibility. This scaffold would be promising
in urinary bladder tissue engineering.
Keywords: PLLA · Electrospun nanofiber · Bladder ·
Tissue engineering
Application: Scaffold, Tissue Engineering
Product Model 1: Electroris
Product Model 2:
URL: https://link.springer.com/article/10.1007/s11255-016-1259-2#="https://link.springer.com" & "/article/10.1007/s11255-016-1259-2"#