ISI Papers With Our Products

Title: Development of an albumin decorated lipid-polymer hybrid nanoparticle for simultaneous delivery of methotrexate and conferone to cancer cells
Journal: International Journal of Pharmaceutics
Author: 1. Gholamreza Dehghan, Mohammad Ali Hosseinpour Feizi, 1,2. Leila Khalili, 2. Hamed Hamishekar, 3. Nader Sheibani
Year: 2021
Address: 1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran 2. Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran 3. Departments of Ophthalmology and Visual Sciences, Cell and Regenerative Biology, and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States
Abstract: Aiming to simultaneous target of methotrexate (MTX), as folate antagonist, and conferone (CON) in various cancer cells, the newly lipid/polymer hybrid nanoparticle containing an albumin targeted succinylchitosan shell and lipoid bilayer core composed of hydrogenated soy phosphatidylcholine and cholesterol was synthesized. The covalently conjugating albumin to the external surface of chitosan was accomplished using N-(3-Dimethylaminopropyl)- N-ethylcarbodiimide hydrochloride and N- hydroxyl succinimide as an activating carboxylic group, and nanoliposomes were fabricated via thin film hydration-sonication method. The molecular structure of MTX@CON-targeted lipid/polymer hybrid nanoparticle (MTX@CON-TLPN) were characterized using FTIR spectroscopy, 1H NMR, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and dynamic light scattering (DLS). The newly nanoparticle with high encapsulation efficiency (85.12%, and 78.4%), acceptable loading capacity (9.8% and 4.6% for MTX and CON) and the stimuli responsiveness drug release behavior in simulated physiologic tumor tissue condition (pH 5.4, 40 ◦C) was successfully synthetized in the spherical shape with mean average size of approximately 290 nm and ζ-potential of +21 mv. The enhanced efficiency of the targeted nanoparticle was further confirmed using MTT endpoints, cell cycle modulation, apoptosis assessment, and cellular internalization assessments. Collectively, these findings establish the utility of our newly prepared nanoparticle for simultaneous delivery of multiple anti-cancer drugs.
Keywords: Conferone, Methotrexate, Hybrid nanoparticle, Targeted delivery
Application: Drug Delivery
Product Model 1: Syringe Pump (SP1000HOM)
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/abs/pii/S0378517321002258#