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Title: Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers
Journal: International Journal of Morphology
Author: 1. Ali Talebi, Shadan Navid, Ayob Jabari, Sepideh Ashouri Movassagh, Farnaz Khadivi, Mehdi Abbasi, 2. Mohammad Ali Sadighi Gilani, 3. Morteza Koruji, 4. Jafar Ai, 5. Mohammad Jafar Rezaie, 6. Majid Salehi, 7. Yumi Hoshino
Year: 2019
Address: 1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran 3. Cellular and Molecular Research Center & Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran 4. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 5. Department of Embryology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran 6. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran 7. Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan
Abstract: Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters.
Keywords: Adult germline stem cell, Male infertility, Cell differentiation, Polycaprolactone, Nanofibers
Application: Scaffold, Tissue Engineering
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